Spectrophotometric Method for the Estimation of 6-Aminopenicillanic Acid

Spectrophotometric method for the estimation of 6-aminopenicillanic acid.

A simple, rapid, and sensitive method is described whereby 6-aminopenicillanic acid can be spectrophotometrically determined in the presence of penicillins and their degradation products without prior separation. d-(+)-Glucosamine is used as reagent. The effect of such parameters as pH, temperature, and time of heating on the formation of the chromophore is described. The recommended range is f...

Spectrophotometric Determination of 6-Aminopenicillanic Acid Using Bromophenol Blue and Bromothymol Blue

Two simple, selective and sensitive spectrophotometric methods are described for the determination of 6-aminopenicillanic acid (6-APA). The methods are based on the reaction of 6-APA with either bromophenol blue (BPB) or bromothymol blue (BTB), to give orange-red and green species, respectively. The coloured products are quantified spectrophotometrically at 625 and 616nm for BPB and BTB, respec...

Preparation of 7-aminocephalosporanic acid and 6-aminopenicillanic acid.

Acylation of 6-Aminopenicillanic Acid by Penicillium chrysogenum.

num deacylated phenoxymethylpenicillin about six times faster than benzylpenicillin to 6-aminopenicillanic acid (6-APA) (R. C. Erickson and R. E. Bennett, Bacteriol. Proc., p. 65, 1961; Erickson and Bennett, Appl. Microbiol. 13:738, 1965. This was observed with a penicillin-nonproducing strain, Wis 49408 (obtained from the University of Wisconsin), and with a penicillinproducing strain, P-5009 ...

Temperature-sensitive 6-aminopenicillanic acid-resistant mutants of Escherichia coli.

Wild-type Escherichia coli cells became spherical in the presence of low concentrations of 6-aminopenicillanic acid (APA). Higher concentrations of APA caused inhibition of cell division (filamentation) and lysis. Spontaneous temperature-sensitive APA-resistant mutants were isolated and characterized. These mutants also possessed increased resistance to mecillinam but not to benzylpenicillin, a...